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The Gut Microbiota and Its Implication in The Development of Atherosclerosis and Related Cardiovascular Diseases

Introduction to Bile Acids Experiment

Patients with cardiovascular diseases significantly excrete lower amounts of bile acid than patients without cardiovascular malfunctions (Sanchez et al., 2020). Therefore, this proposition develops the hypothesis that the inability to excrete adequate bile acids leads to cardiovascular diseases. This experiment investigates the long term role of bile acid excretion in the development of cardiovascular diseases and its relation to 10 patients with a proven cardiovascular infection compared with other ten patients without cardiovascular disease matched for similar laboratory features. The results are then analyzed for informed conclusions that will either accept or reject the hypothesis by proof.


H0: Inability to excrete adequate bile acids lead to the development of cardiovascular diseases

H1: Inability to excrete adequate bile acids does not lead to the development of cardiovascular diseases


The patients in the sample size of 20 patients are fed with a standard diet for five days. The diet includes 500 milligrams of cholesterol. Fecal from bile acids from stool is collected after 24 hours and measured through a gas-liquid chromatogram.


The patients with cardiovascular disease excreted lower amounts of lethocholic acids (p<0.01), and bile acids and deoxycholic acids (p<0.001) than the controls. Bile Acids Excretion is an independent factor and predicted cardiovascular diseases (p<0.05).


The result proofs that patients with cardiovascular diseases had decreased bile acid excretion compared to the controls. Therefore, this provides adequate evidence to accept the null hypothesis.

References for Bile Acids Experiment

Sanchez-Rodriguez, E., Egea-Zorrilla, A., Plaza-Díaz, J., Aragón-Vela, J., Muñoz-Quezada, S., Tercedor-Sánchez, L., & Abadia-Molina, F. (2020). The Gut Microbiota and Its Implication in the Development of Atherosclerosis and Related Cardiovascular Diseases. Nutrients12(3), 605.

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