Dataset description: Investigation of burn injuries in a porcine model using metabolomics
Burn injury initiates a hypermetabolic response that leads to muscle wasting and organ dysfunction in burn patients. The lab examined the transition between “ebb” and “flow” in post-burn metabolism using proton nuclear magnetic resonance (1H-NMR) spectroscopy and serum from a porcine model of severe burn injury. We hypothesized serum metabolomes of porcine subjects would be distinguishable by time point, and changes in individual metabolite concentrations would characterize the shift from ebb to flow after burns. Methods: Pigs received 40% total body surface area (TBSA) burns with additional pine bark smoke inhalation. Arterial blood was drawn at baseline (pre-burn, “control” samples) and 48h (“treated” samples) post injury. The aqueous portion of each serum sample was analyzed using 1H-NMR spectroscopy.
Task: please analyse together the dataset that you received via email by answering to the three parts, each of them described below and corresponding to a specific analysis in MetaboAnalyst. Be as specific as possible, and don't forget to put the figures whenever requested.
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